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Second, although ARPE-19 cells are a common model for studying retinal function [68, 69], their standard culture medium already contains elevated glucose levels higher than physiological norms

[5] This reasoning motivated the human withdrawal studies discussed later

Cell culture analyses The human colon cancer cell line HCT116 and human hepatoma cell line HuH7 were maintained in DMEM medium containing 10% FCS

However, transporter involvement in physiologically relevant BBB models remains to be mechanistically confirmed, warranting further validation in mammalian systems